PCR + Culture
Methods
<10 CFU/mL
Sensitivity
All Species
Coverage
3–7 days
TAT

Why Mycoplasma Testing Is Essential for Cell Culture

Mycoplasma contamination is the most common and most destructive quality issue in cell culture — affecting >30% of cell lines at some institutions.

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Invisible Contamination

Mycoplasma lack cell walls and don't cloud media, making them invisible without dedicated testing.

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PCR Speed

PCR-based detection provides same-day or next-day results for rapid lot release decisions.

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Biologics Production

Cell lines used in biologics manufacturing must demonstrate mycoplasma-free status. We provide documentation for GMP support.

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Cell Therapy

CAR-T and other cell therapy programs require mycoplasma testing at multiple manufacturing stages.

Mycoplasma Testing Scope

PCR for rapid screening, culture for regulatory-grade confirmation.

How to Submit a Sample

Submitting takes less than 5 minutes. Most projects turn around in 5–10 business days from sample receipt.

1

Request Online

Fill out our sample intake form — sample type, quantity, and timeline. We confirm turnaround and quote within one business day.

2

Ship Your Sample

We'll send submission instructions and our San Diego shipping address. Most samples ship ambient or cold depending on stability requirements.

3

Receive Your COA

Results are delivered as a signed PDF COA within the agreed turnaround. Rush service (2–3 business days) available for urgent timelines.

Request a Test →

Frequently Asked Questions

What is the difference between PCR and culture-based mycoplasma testing?

PCR provides rapid results (1–2 days) and high sensitivity but cannot distinguish viable from non-viable organisms. Culture-based testing (14–28 days) is considered the gold standard for regulatory submissions and detects viable organisms only.

Do you perform both PCR and culture?

Yes. We offer PCR-only, culture-only, or combined testing depending on your timeline and regulatory requirements.

What sample types can be tested?

Cell-conditioned media, culture supernatant, cell lysates, and finished biologics are all acceptable. Contact us if you have an unusual matrix.

Ready to Get Your COA?

Submit your samples and receive an ISO 17025 accreditation pending Certificate of Analysis. Results in 5–10 business days.